Topo vector invitrogen
WebResistant to Kanamycin (KanR) and Zeocin™ (ZeoR). pCR™ 2.1-TOPO; TA vector: cloning and subcloning simplicity The pCR 2.1-TOPO TA vector is linearized with 3'-thymidine (T) overhangs for direct ligation of Taq polymerase–amplified PCR products (TA cloning) and is “activated” with covalently bound Topoisomerase I. There is no need to ... WebpENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry vector for the Gateway® System Catalog numbers K2400-20, K2420-20, K2525-20, K2535-20, K2435-20, and K2635-20 Revision Date 29 March 2012 Publication part number 25-0434 MAN0000245
Topo vector invitrogen
Did you know?
WebInvitrogen TOPO PCR cloning technology was developed to help improve cloning efficiency, simplify protocol setup, and accommodate a wide range of PCR insert sizes. TOPO … Verifying your credentials.. Please do not close this window. Order now. Plate oligos . Scales, lengths, purifications, modifications: Same as … Invitrogen SuperScript IV products aid in exceptional cDNA synthesis from any … WebComments for pCR®-Blunt II-TOPO® 3519 nucleotides lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-576 SP6 promoter priming site: bases 239-256 Multiple Cloning Site: bases 269-399 TOPO®-Cloning site: bases 336-337 T7 promoter priming site: bases 406-425
WebI have cloned the gene in TOPO TA cloning vector from invitrogen. Now I am doing LR reaction with LR Clonase™ II Plus enzyme from invitrogen. My insert size is 1374bp and destination vector ... WebThe U.S. Geological Survey (USGS) has been the primary civilian mapping agency of the United States since 1879. USGS topographic maps have been published at many scales, …
WebDescription. The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts. Fast and convenient: 15-minute, room-temperature ligation. Efficient: blue/white screening and >80% ... WebDirectional TOPO® cloning vector, for creating a Gateway® entry vector that is suitable for recombination with a destination vector. Sequence Author: Thermo Fisher (Invitrogen) …
WebInvitrogen Corporation 1600 Faraday Avenue Carlsbad, CA 92008 T: 1 760 603 7200 F: 1 760 602 6500 E: [email protected] For country-specific contact information visit our web site at www.invitrogen.com pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry vector
WebI have cloned the gene in TOPO TA cloning vector from invitrogen. Now I am doing LR reaction with LR Clonase™ II Plus enzyme from invitrogen. My insert size is 1374bp and destination vector ... new tattoo urbandub lyricsWebOct 12, 2024 · I did a Topo reaction to get a 3kb insert into 2.5kb pENTR/D/Topo vector and transformed into Invitrogen Top10 Chemically Competent cells. The cells have an option for blue/white screening after ... new tatumWebThis means sequencing reactions give less vector sequence and more insert sequence. The pCR4-TOPO TA vector has sites for 4 common sequencing primers: M13 forward, M13 reverse, T7, and T3. The kits include an aliquot of each. The pCR4-TOPO TA vector contains both ampicillin and kanamycin resistance markers and a LacZα-ccdB gene fusion for ... midstream investment banking analystWebpCR-XL-TOPO® allows direct selection of recombinants via disruption of the lethal E. coli gene, ccdB (Bernard et al., 1994). The vector contains the B gene fused to the C-terminus of the LacZα fragment. Ligation of a long PCR product disrupts expression of the lacZα-ccdB gene fusion permitting growth of only new tattoo show on tvWebBacterial directional TOPO® cloning vector with an ampicillin resistance marker, for inducible expression of an N-terminally 6xHis-tagged protein. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Try SnapGene for Free. Download Plasmid Download SnapGene Viewer. Explore Over 2.7k Plasmids: TOPO Cloning Vectors More … new tattoo sunscreenWebMar 23, 2012 · TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes new tattoo urbandubWebAbstract. We evaluated the cloning efficiency of different size PCR products into three T-vector cloning systems. The pGEM®-T and pGEM®-T Easy Vector Systems gave a high number of recombinants across a broad range of insert sizes (0.5–3kb) while the TOPO TA Cloning® system worked well for inserts less than 1kb, but showed a striking decrease in … new tatumchester