WebTo examine the intracellular transport of transmembrane proteins to and from the cell surface, cell-surface biotinylation is a proven powerful method. Using this method, we successfully demonstrated that occludin was endocytosed and recycled back to the cell surface in both fibroblastic baby hamster kidney (BHK) and epithelial MTD-1A cells.

Biotin labeling and quantitation of cell-surface proteins

WebJan 1, 2014 · Biotinylation recycling assay. ( a) Cells were serum starved overnight and then cell-surface proteins were biotinylated at 4 °C, before returning the cells to 37 °C with medium containing ligand for 15 min. Cells were cooled to 4 °C and the remaining surface biotin was stripped with MeSNa buffer. WebBiotinylation reagents for the study of cell surface proteins. The extraordinarily stable, non-covalent interaction between avidin and biotin is one of the most commonly exploited … rally marshal

Biotinylation Thermo Fisher Scientific - US

WebThe OCW Assay can be used to: Detect and quantify target proteins localized to the cell surface; Quantify ligand binding to cell surface receptors; Monitor receptor internalization and recycling by detecting loss … WebApr 22, 2011 · Because the difference in internalization rate of β1 integrin between stiff and soft substrates may have resulted from different recycling rates, a surface biotinylation assay was performed in the presence of primaquine (PMQ), a well-established reversible inhibitor of receptor recycling (18, 19). PMQ did not affect the internalization of β1 ... WebCell-surface biotinylation assay to detect the surface-expressed receptors (Suh et al. Citation 2010) revealed that the surface-to-total expression level of SEP-GluA1 was not altered by a 5-min treatment with bafilomycin A1 (2 μM), where the SEP fluorescence was increased in the live-cell imaging (Figure 1A), compared to untreated control (0 ... rally maremma 2023