Web13 nov. 2006 · The direct effect of the rotavirus NSP4 114-135 and Norovirus NV 464-483 peptides on 36 Cl uptake was studied by using villus cell brush border membrane (BBM) isolated from young rabbits. Both peptides inhibited the Cl-/H + symport activity about equally and partially. The interaction involved one peptide-binding site per carrier unit. … Web12 mrt. 2011 · The nonstructural protein NSP4 acts as a rotavirus enterotoxin, inducing secretory diarrhea without any structural organ damage. Electrolyte transport was assessed in the colonic epithelium from pups and adult mice using Ussing chamber recordings.
Rotavirus NSP4: A Multifunctional Viral Enterotoxin
WebA transmembrane glycoprotein, NSP4 is organized into three main domains: a three-helical TM domain in the N-terminus (also a viroporin domain), a central cytoplasmic coiled-coil domain for multimerization, and an C-terminal flexible region. It can also be secreted … Web24 jan. 2006 · The castor-oil plant Ricinus communis A–B dimeric toxin B subunit (RTB) was genetically linked at its N-terminus with a 90 amino acid peptide from simian rotavirus SA-11 non-structural protein NSP4 90 and produced in Escherichia coli BL21 cells. Biologically active recombinant NSP4 90 –RTB fusion protein was shown to bind … china education group hong kong limited
Oral immunization with a shiga toxin B subunit: rotavirus NSP4(90 ...
Web12 mei 2024 · NSP4a–NSP4c were created by modifying the h-region of NSP4 by changing the position of amino acid residues or substituting residues with polyleucine or polyalanine. d Set III novel signal peptides. Web1 okt. 2005 · Oral immunization with a shiga toxin B subunit: rotavirus NSP4(90) fusion protein protects mice against gastroenteritis. Nak-Won Choi Center for Molecular Biology … WebThe Shiga toxin (a non-pore forming toxin) is transferred to the cytosol via Golgi network and endoplasmic reticulum (ER). From the Golgi toxin is trafficked to the ER. Shiga toxins act to inhibit protein synthesis within … china education daily