Cy3 conjugation
WebCy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG Clonality Polyclonal WebMay 20, 2024 · Step 2. Add Reagents To Reaction Mixture. Dry EDC, alongside NHS or sulfo-NHS if used to stabilize O-acylisourea, are added to the amine-containing solution in the conjugation buffer for 15 minutes at room temperature. Step 3. Quench EDC Coupling Reaction. A thiol-containing compound such as 2-mercaptoethanol is added.
Cy3 conjugation
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WebSignalSilence ® Control siRNA (Cy3 ® Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a … Cyanines, also referred to as tetramethylindo(di)-carbocyanines are a synthetic dye family belonging to the polymethine group. Although the name derives etymologically from terms for shades of blue, the cyanine family covers the electromagnetic spectrum from near IR to UV. Chemically, cyanines are a conjugated system between two nitrogen atoms; in each resonance structure, exactly one nitrogen atom is oxidized to an iminium. Typically, they form part of a nitro…
WebSecondary antibodies can be conjugated to enzymes, biotin, and fluorescent dyes/proteins. The conjugate choice depends upon the experimental application and allows colorimetric, fluorescent, or chemiluminescent detection of primary antibodies in cell imaging, immunohistochemistry, flow cytometry, and western blotting. WebConjugation: PE-Cy3: Host: Rabbit: Source: KLH conjugated synthetic peptide derived from human IP3 Receptor: Immunogen Range: 101-200/2758: Clonality: Polyclonal: Isotype: IgG: Concentration: 1ug/ul: Purification: Purified by Protein A. Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and …
WebOct 4, 2024 · The primary mechanism of conjugation consists of 1,4 addition of the amine to maleimide which ketonizes to the final product (see image below). By increasing … WebCy™3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy™3 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, …
WebAnti-GFAP Antibody, Cy3 Conjugate. Compare Product No. Clonality Application Species Reactivity Citations SDS; MAB3402C3: monoclonal: ICC, IHC: mouse, pig: 10: Expand. Hide. Match Criteria: Product Name. All Photos (1) Monoclonal Anti-Actin–Cy3 antibody produced in mouse. Synonym(s): Monoclonal Anti-Actin. Compare Product No. Clonality
WebOct 29, 2013 · Abcam’s Cy3® Conjugation Kit provides a simple and quick process to conjugate your primary antibodies with Cy3®. Cy3® is a yellow fluorescent dye which … canon ink pg-275 cl-276WebGeneral notes. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. The product is prepared by conjugation of Cy3 to purified monoclonal anti-beta-tubulin antibody. The conjugate is purified by gel filtration to remove unbound Cy3 fluorophore. F/P Molar Ratio: (Cy3:Ab) 3 to 9. canon ink recycle programWebHow are the Alexa Fluor dyes better in performance than their standard counterparts such as fluorescein (FITC), Cy3, Cy5, Texas Red, and allophycocyanin (APC) dyes? Do I need to buy new emission filters to spectrally match the Alexa Fluor dyes? What is the pH stability of Alexa Fluor dyes? What is the usable pH range of Alexa Fluor dyes? Are Alexa Fluor … flagship login for auto paymentsWebCy5 ® Conjugation Kit / Cy5 ® Labeling Kit (ab188288) uses a simple and quick process for Cy5 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages. The Cy5 conjugated antibody can be used immediately in WB, ELISA, IHC etc. canon ink pg540 cl541canon ink pgbk 270WebOverview: Catalog # bs-7068R-Cy3: Product Name: FOXO1/3/4 Polyclonal Antibody, Cy3 Conjugated: Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC) Reactivity flagship location meaningWeb瑞禧生物提供科研级Cy3 Alkyne/Cyanine3 alkyne,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。Cy3 Alkyne/Cyanine3 alkyne试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购Cy3 Alkyne/Cyanine3 alkyne，请联系我们：18966759382 canon ink pg-545xl